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Article

GP5+/6+ PCR followed by reverse line blot analysis enables rapid and high-throughput identification of human papillomavirus genotypes.

Journal of clinical microbiology - 1 Mar 2002

van den Brule Adriaan J C, Pol René, Fransen-Daalmeijer Nathalie, Schouls Leo M, Meijer Chris J L M, Snijders Peter J F

Abstract excerpt

In this study, we developed a simple and fast typing procedure for 37 mucosotropic human papillomavirus (HPV) types using a nonradioactive reverse line blotting (RLB) procedure for general primer (GP5+/6+) PCR products. This system has the advantages not only that in a simple format, up to 42 PCR products can be simultaneously typed per membrane per day, but also that after stripping, the membranes can be easily...

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Identifiers

PMID: 11880393
DOI: 10.1128/JCM.40.3.779-787.2002
PMCID: PMC120256

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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