Article
Head-to-head comparison of genotyping of human papillomavirus by real-time multiplex PCR assay using type-specific primers and SPF10-PCR-based line probe assay.
Journal of medical virology - 1 Feb 2023
Yin Jian, Peng Siying, Zhang Changning, Li Xinyue, Hu Fangfang, Chen Wen, Qiao Youlin
Abstract excerpt
The SPF10-polymerase chain reaction (PCR)-based line probe assay (LiPA-25) with high analytical sensitivity and specificity for human papillomavirus (HPV) genotyping in clinical samples has been widely used in vaccine and epidemiologic studies. A real-time multiplex PCR assay using type-specific primers (Hybribio-23) with low workload and cost has been developed recently. The study aimed to compare the...
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