Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genotyping of 27 human papillomavirus types by using L1 consensus PCR products by a single-hybridization, reverse line blot detection method.

Journal of clinical microbiology - 1 Oct 1998

Gravitt P E, Peyton C L, Apple R J, Wheeler C M

Abstract excerpt

Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (e.g., MY09/11 or GP5(+)/6(+)) can detect as few as 10 to 100 molecules of HPV targets from a genital sample. However, genotype determination by dot blot hybridization is laborious and requires at least 27 separate hybridizations for substantive HPV-type discrimination. A reverse blot method was developed which employs a biotin-labeled...

Topics

Animals
Base Sequence
Biotinylation
Cattle
Cervix Uteri
Consensus Sequence
Female
Genotype
Humans
Oligonucleotide Probes
Papillomaviridae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Papillomavirus Infections

Polymerase Chain Reaction

Random Amplified Polymorphic DNA Technique

Sensitivity and Specificity

Tumor Virus Infections

Source

Open in PubMed Open full text

Identifiers

PMID: 9738060
DOI: 10.1128/JCM.36.10.3020-3027.1998
PMCID: PMC105104

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-9738060-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genotyping of 27 human papillomavirus types by using L1 consensus PCR products by a single-hybridization, reverse line blot detection method.PMID 9738060Read ↗

Development and Clinical Evaluation of a Highly Sensitive PCR-Reverse Hybridization Line Probe Assay for Detection and Identification of Anogenital Human PapillomavirusSemantic proximity Read ↗

Comparison of Human Papillomavirus Detection and Typing by Cycle Sequencing, Line Blotting, and Hybrid CaptureSemantic proximity Read ↗

Evaluation of Different Techniques for Identification of Human Papillomavirus Types of Low PrevalenceSemantic proximity Read ↗

Comparison of the performance of different HPV genotyping methods for detecting genital HPV types.Semantic proximity Read ↗

Head-to-head comparison of genotyping of human papillomavirus by GP5+/6+-PCR-based reverse dot blot hybridization assay and SPF10-PCR-based line probe assay.Semantic proximity Read ↗

Detection and typing of human papillomaviruses combining different methods: polymerase chain reaction, restriction fragment length polymorphism, line probe assay and sequencing.Semantic proximity Read ↗

Comparison of Linear Array and Line Blot Assay for Detection of Human Papillomavirus and Diagnosis of Cervical Precancer and Cancer in the Atypical Squamous Cell of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage StudySemantic proximity Read ↗

GP5+/6+ PCR followed by reverse line blot analysis enables rapid and high-throughput identification of human papillomavirus genotypes.Semantic proximity Read ↗

Genotyping of human papillomavirus in cervical lesions by L1 consensus PCR and the Luminex xMAP system.Semantic proximity Read ↗

Human papillomavirus typing with a polymerase chain reaction-based genotyping array compared with type-specific PCR.Semantic proximity Read ↗

Analytical performance of Cervista HPV 16/18 genotyping test for cervical cytology samples.Semantic proximity Read ↗

Human papillomavirus genotype specificity of hybrid capture 2.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.