Article
[Cloning whole length cDNA of related genes responsible for smooth muscle cells proliferation in atherogenesis and study on its function].
Zhonghua bing li xue za zhi = Chinese journal of pathology - 1 Oct 1999
Zhao G, Gao P, Zhang H, Lou L, Qiao H, She M
Abstract excerpt
OBJECTIVE: To clone whole length cDNA of the related genes responsible for vascular smooth muscle cell (SMC) proliferation in atherogenesis, and to study its function. METHODS: ox-LDL was added as a stimulant to the SMC culture medium. Subtractive library was established using subtractive hybridization technique in order to clone the related genes fragments. With the whole length cDNA library established, the...
Topics
- Arteriosclerosis
- Cell Division
- Cloning, Molecular
- DNA, Complementary
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Gene Expression
- Genetic Predisposition to Disease
- Humans
- Lipoproteins, LDL
