Binding specificity and the ligand dissociation process in the E. coli biotin holoenzyme synthetase.

Abstract excerpt

The binding of the Escherichia coli biotin holoenzyme synthetase to the two ligands, biotin and bio-5'-AMP, is coupled to disorder-to-order transitions in the protein. In the structure of the biotin complex, a "glycine-rich" loop that is disordered in the apo-enzyme is folded over the ligand. Mutations in three residues in this loop result in significant changes in the affinity of the enzyme for both biotin and...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Adenosine Monophosphate
• Share to Amino Acid Substitution