Plasmodium falciparum: underestimation of dihydrofolate reductase and dihydropteroate synthase polymorphism in field samples: a technical shortcoming of nested PCR assays with mutation-specific primers.

Abstract excerpt

n a study parallel to the present one, we conducted a genotype characterization of Plasmodium falciparum based on isolates of patients infected with malaria, who come from a small location in Colombia. The analysis involved extraction of DNA from hematological smears, amplification of the dihydrofolate reductase gene and the dihydropteroate synthase (DHPS) gene for each sample by PCR, and detection through...

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