High‐level expression of human thyroid‐stimulating hormone in Chinese hamster ovary cells by co‐transfection of dicistronic expression vectors followed by a dual‐marker amplification strategy

Abstract excerpt

The utilization of dicistronic mRNA expression vectors, containing the gene of interest upstream of an amplifiable marker gene, has shown success in rapidly, efficiently and reproducibly obtaining stable cell lines that express high levels of the protein of interest. For this reason, human thyroid-stimulating hormone (hTSH), a heterodimeric glycoprotein composed of non-covalently linked alpha- and beta-subunits,...

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