Article
Expression of a human serum albumin variant with high affinity for thyroxine.
Biochemical and biophysical research communications - 25 Sept 1995
Petersen C E, Ha C E, Mandel M, Bhagavan N V
Abstract excerpt
In this study a protein expression system was used to synthesize recombinant human serum albumin containing a mutation that has been shown to result in familial dysalbuminemic hyperthyroxinemia. Equilibrium dialysis was used to measure the binding of this recombinant human serum albumin with thyroxine. The association constant determined for the binding of this human serum albumin variant with thyroxine was shown...
Topics
- Amino Acid Sequence
- Binding Sites
- Cloning, Molecular
- Gene Expression
- Genetic Diseases, Inborn
- Genetic Variation
- Humans
- Kinetics
- Liver
- Molecular Sequence Data
- Mutation