Article
Kinetic characterization of ribonuclease S mutants containing photoisomerizable phenylazophenylalanine residues.
Protein engineering - 1 Dec 2001
James D A, Burns D C, Woolley G A
Abstract excerpt
Incorporation of the photoisomerizable amino acid phenylazophenylalanine (PAP) into enzyme structures has been proposed as a strategy for photoswitching enzyme activity. To evaluate the strengths and limitations of this approach to enzyme photo-control, we performed a kinetic analysis of RNase S analogues containing PAP in positions 4, 7, 8, 10, 11 or 13. For an enzyme containing a single PAP group, the maximum...
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