Article
Implication of a novel multiprotein Dam1p complex in outer kinetochore function.
The Journal of cell biology - 24 Dec 2001
Cheeseman I M, Brew C, Wolyniak M, Desai A, Anderson S, Muster N, Yates J R, Huffaker T C, Drubin D G, Barnes G
Abstract excerpt
Dam1p, Duo1p, and Dad1p can associate with each other physically and are required for both spindle integrity and kinetochore function in budding yeast. Here, we present our purification from yeast extracts of an approximately 245 kD complex containing Dam1p, Duo1p, and Dad1p and Spc19p, Spc34p, and the previously uncharacterized proteins Dad2p and Ask1p. This Dam1p complex appears to be regulated through the...
Topics
- Antigens, Neoplasm
- Cell Cycle Proteins
- Cytoskeletal Proteins
- Fluorescent Antibody Technique
- Fungal Proteins
- Genotype
- Green Fluorescent Proteins
- Kinetochores
- Luminescent Proteins
- Macromolecular Substances
- Mass Spectrometry
- Microtubules
- Multiprotein Complexes