Article
Detection of anti-p53 antibodies by ELISA using p53 synthetic or phage-displayed peptides.
Journal of immunological methods - 1 Jan 2002
Portefaix Jean Michel, Fanutti Cristina, Granier Claude, Crapez Evelyne, Perham Richard, Grenier Jean, Pau Bernard, Del Rio Maguy
Abstract excerpt
Anti-p53 antibodies have been detected in the sera of patients with various types of cancers. In this report, we describe the development of a new ELISA aimed at detecting anti-p53 antibodies using two peptides belonging to immunodominant epitopes of the p53 N-terminal region. We first tested the reactivity of the sera by an indirect ELISA using the peptides as a capture system. Then, the specificity of the...
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