The effect of mosquito passage on the La Crosse virus genotype.

doi:10.1099/0022-1317-82-12-2919

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Article

The effect of mosquito passage on the La Crosse virus genotype.

The Journal of general virology - 1 Dec 2001

Borucki Monica K, Kempf Brian J, Blair Carol D, Beaty Barry J

Abstract excerpt

The genetic consequences of passing three different strains of La Crosse (LAC) virus orally and transovarially in Aedes triseriatus mosquitoes were examined. Two of the LAC strains (WT LAC and LAC ORI) had been passaged numerous times in cell culture; the third strain (SM1-78) had been passaged only once in suckling mice. Genetic changes were monitored in three regions of the LAC genome after oral infection and...

Topics

Amino Acid Substitution
Animals
Cloning, Molecular
Culicidae
Female
Genotype
Intestines
La Crosse virus
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Ovary
Viral Proteins
Virus Replication

Source

Open in PubMed

Identifiers

PMID: 11714967
DOI: 10.1099/0022-1317-82-12-2919

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