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Detection of Cryptosporidium parvum DNA in human feces by nested PCR

1 Jul 1996

Abstract excerpt

Cryptosporidium parvum is a coccidian protozoan that causes diarrhea in humans, often chronic and severe in patients with AIDS. Conventionally, diagnosis is made by concentration of stools followed by acid-fast staining (AF) or immunofluorescent staining. The threshold of detection in human stool specimens by these methods may require the presence of 50,000 (immunofluorescent staining) to 500,000 (AF) oocysts per...

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PMID: 8784586
DOI: 10.1128/jcm.34.7.1769-1772.1996

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