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Detection and identification by real time PCR/RFLP analyses of Cryptosporidium species from human faeces

12 Feb 2004

Abstract excerpt

AIMS: To detect a wide range of Cryptosporidium species from human faeces by analysis of the Cryptosporidium oocyst wall protein gene by PCR. METHODS AND RESULTS: The nested-assay comprised an initial amplification using a conventional thermocycler followed by real time PCR using a LightCycler with SYBR Green I for the characterization of the amplicons. The technique uses four sets of primers composed of five to...

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PMID: 14962043
DOI: 10.1111/j.1472-765x.2004.01473.x

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