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Increased Birefringence in the Meiotic Spindle Provides a New Marker for the Onset of Activation in Living Oocytes1

1 Jul 2000

Abstract excerpt

The newly developed Pol-Scope allows imaging of spindle retardance, which is an optical property of organized macromolecular structures that can be observed in living cells without fixation or staining. Experiments were undertaken to examine changes in meiotic spindles during the initial stages of activation of living mouse oocytes using the Pol-Scope. Parthenogenetic activation of oocytes treated with calcium...

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PMID: 10859266
DOI: 10.1095/biolreprod63.1.251

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