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Article

Effects of Cooling and Rewarming on the Meiotic Spindle and Chromosomes of in Vitro-Matured Bovine Oocytes1

1 Jan 1994

Abstract excerpt

The purpose of this study was to evaluate effects of cooling and rewarming on the meiotic spindle apparatus of bovine oocytes. In experiment 1, in vitro-matured bovine oocytes were either maintained at 39 degrees C or cooled abruptly to 4 degrees C or approximately 25 degrees C. Immunohistochemical and DNA staining for visualization of microtubules and chromosomes, respectively, revealed an anastral,...

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PMID: 8312433
DOI: 10.1095/biolreprod50.1.103

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