Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag.

doi:10.1371/journal.pone.0016226

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Article

Stabilization by fusion to the C-terminus of hyperthermophile Sulfolobus tokodaii RNase HI: a possibility of protein stabilization tag.

PloS one - 19 Jan 2011

Takano Kazufumi, Okamoto Tomohiro, Okada Jun, Tanaka Shun-ichi, Angkawidjaja Clement, Koga Yuichi, Kanaya Shigenori

Abstract excerpt

RNase HI from the hyperthermophile Sulfolobus tokodaii (Sto-RNase HI) is stabilized by its C-terminal residues. In this work, the stabilization effect of the Sto-RNase HI C-terminal residues was investigated in detail by thermodynamic measurements of the stability of variants lacking the disulfide bond (C58/145A), or the six C-terminal residues (ΔC6) and by structural analysis of ΔC6. The results showed that the...

Topics

Amino Acid Sequence
Genetic Variation
Methods
Protein Stability
Recombinant Fusion Proteins
Ribonuclease H
Sulfolobus
Thermodynamics

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Identifiers

PMID: 21283826
DOI: 10.1371/journal.pone.0016226
PMCID: PMC3023800

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