Article
Reconstruction of Escherichia coli mrcA (PBP 1a) mutants lacking multiple combinations of penicillin binding proteins.
Journal of bacteriology - 1 Oct 2001
Meberg B M, Sailer F C, Nelson D E, Young K D
Abstract excerpt
Previously, we constructed a set of mutants from which eight penicillin binding protein (PBP) genes were deleted in 192 combinations from Escherichia coli (S. A. Denome, P. K. Elf, T. A. Henderson, D. E. Nelson, and K. D. Young, J. Bacteriol. 181:3981-3993, 1999). Although these mutants were constructed correctly as determined by restriction mapping and the absence of relevant protein products, we recently...
Topics
- Bacterial Proteins
- Carrier Proteins
- Escherichia coli
- Genes, Bacterial
- Hexosyltransferases
- Multienzyme Complexes
- Muramoylpentapeptide Carboxypeptidase
- Mutation
- Penicillin-Binding Proteins
- Peptidyl Transferases
- Phenotype
