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Article

Expression of yeast L-A double-stranded RNA virus proteins produces derepressed replication: a ski- phenocopy.

Journal of virology - 1 Jan 1991

Wickner R B, Icho T, Fujimura T, Widner W R

Abstract excerpt

The plus strand of the L-A double-stranded RNA virus of Saccharomyces cerevisiae has two large open reading frames, ORF1, which encodes the major coat protein, and ORF2, which encodes a single-stranded RNA-binding protein having a sequence diagnostic of viral RNA-dependent RNA polymerases. ORF2 is expressed only as a Gag-Pol-type fusion protein with ORF1. We have constructed a plasmid which expresses these...

Topics

Capsid
DNA-Directed RNA Polymerases
Escherichia coli
Genes, Viral
Genetic Vectors
Genotype
Mutagenesis
Open Reading Frames
Plasmids
RNA Viruses
RNA, Double-Stranded

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Restriction Mapping

Saccharomyces cerevisiae

Suppression, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 1985195
DOI: 10.1128/JVI.65.1.155-161.1991
PMCID: PMC240500

Genes

PGK1
SKI

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1985195-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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