Article
Attenuation of a Brucella abortus mutant lacking a major 25 kDa outer membrane protein in cattle.
American journal of veterinary research - 1 Sept 2001
Edmonds M D, Cloeckaert A, Booth N J, Fulton W T, Hagius S D, Walker J V, Elzer P H
Abstract excerpt
OBJECTIVE: To determine the virulence of a Brucella abortus mutant, BA25, lacking a major 25 kd outer membrane protein (Omp25) in cattle. ANIMALS: 20 mixed-breed heifers in late gestation. PROCEDURE: 10 heifers were inoculated with 1 x 10(7) colony-forming units of the Omp25 mutant via the conjunctival sac, and an equal number were infected with the virulent parental strain B. abortus 2308. The delivery status of...
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