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Cloned pigs generated from cultured skin fibroblasts derived from a H‐transferase transgenic boar

31 Aug 2001

Abstract excerpt

Cloned pigs were produced from cultured skin fibroblasts derived from a H-transferase transgenic boar. One 90 day fetus and two healthy piglets resulted from nuclear transfer by fusion of cultured fibroblasts with enucleated oocytes. The cells used in these studies were subjected to an extensive culture time, freezing and thawing, and clonal expansion from single cells prior to nuclear transfer. PCR and FACS...

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PMID: 11553917
DOI: 10.1002/mrd.1076

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