Article
One-step detection and genotyping of human papillomavirus in cervical samples by reverse hybridization.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Sept 2001
Laconi S, Greco M, Pellegrini-Bettoli P, Rais M, Laconi E, Pani P
Abstract excerpt
This study describes a nonisotopic polymerase chain reaction-reverse hybridization-based method (PCR-RH) for the one-step detection and genotyping of anogenital human papillomavirus (HPV) in a microwell format. HPV DNA was amplified and labeled by PCR using GP5+/GP6+ primers. Labeled amplicons were hybridized to 20 HPV type-specific capture probes anchored to the surface of plastic microwells and detected by an...
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