Article
One-step detection and genotyping of human papillomavirus in cervical samples by reverse hybridization.
Diagnostic molecular pathology : the American journal of surgical pathology, part B - 1 Sept 2001
Laconi S, Greco M, Pellegrini-Bettoli P, Rais M, Laconi E, Pani P
Abstract excerpt
This study describes a nonisotopic polymerase chain reaction-reverse hybridization-based method (PCR-RH) for the one-step detection and genotyping of anogenital human papillomavirus (HPV) in a microwell format. HPV DNA was amplified and labeled by PCR using GP5+/GP6+ primers. Labeled amplicons we...
Topics
- Consensus Sequence
- DNA Primers
- DNA, Viral
- Female
- Genotype
- Humans
- Nucleic Acid Hybridization
- Papillomaviridae
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sensitivity and Specificity
- Uterine Cervical Neoplasms
- Uterine Cervical Dysplasia