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Article

Evaluation of a novel highly sensitive, broad-spectrum PCR-reverse hybridization assay for detection and identification of beta-papillomavirus DNA.

Journal of clinical microbiology - 1 May 2006

de Koning Maurits, Quint Wim, Struijk Linda, Kleter Bernhard, Wanningen Patrick, van Doorn Leen-Jan, Weissenborn Sönke Jan, Feltkamp Mariet, ter Schegget Jan

Abstract excerpt

Human papillomavirus can be detected by amplification of viral DNA. A novel one-step PCR (PM-PCR) was evaluated for amplification of a 117-bp fragment from the E1 region. It permitted ultrasensitive detection of all 25 known human papillomavirus genotypes from the beta-papillomavirus genus. The intra- and intertypic sequence variations of the 77-bp interprimer region were studied. Genotype-specific probes as well...

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Identifiers

PMID: 16672409
DOI: 10.1128/JCM.44.5.1792-1800.2006
PMCID: PMC1479195

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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