Article
Bulk production and functional analyses of mouse CD55's native and deglycosylated active domains.
Archives of biochemistry and biophysics - 1 Sept 2001
Lin F, Immormino R M, Shoham M, Medof M E
Abstract excerpt
We report the use of methylotrophic yeast Pichia pastoris as a host to efficiently express complement control protein repeats (CCPs) 1-4 of mouse decay accelerating factor (DAF, CD55) as a soluble protein. With this system, the mouse DAF CCP1-4-active-domain-containing module linked to a 6x His tag at its C terminus was secreted into the culture supernatant at 15 mg/L after 24 h of induction with methanol. A...
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