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Article

Mammalian ferrochelatase. Expression and characterization of normal and two human protoporphyric ferrochelatases.

The Journal of biological chemistry - 7 Jan 1994

Dailey H A, Sellers V M, Dailey T A

Abstract excerpt

Ferrochelatase (EC 4.99.1.1) catalyzes the terminal step in the heme biosynthetic pathway, the insertion of ferrous iron into protoporphyrin IX. Herein we report the expression, purification, and characterization of the mature processed form of human and mouse ferrochelatase in Escherichia coli JM109. Metal analysis of the recombinant normal human ferrochelatase reveals that there are approximately 2 iron...

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PMID: 8276824

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