Article
Mammalian ferrochelatase. Expression and characterization of normal and two human protoporphyric ferrochelatases.
The Journal of biological chemistry - 7 Jan 1994
Dailey H A, Sellers V M, Dailey T A
Abstract excerpt
Ferrochelatase (EC 4.99.1.1) catalyzes the terminal step in the heme biosynthetic pathway, the insertion of ferrous iron into protoporphyrin IX. Herein we report the expression, purification, and characterization of the mature processed form of human and mouse ferrochelatase in Escherichia coli J...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- DNA Primers
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Ferrochelatase
- Humans
- Kinetics
- Mice
- Molecular Sequence Data
- Mutation
- Plasmids
- Porphyrins
- Recombinant Proteins
- Sequence Homology, Amino Acid