Article
Efficient gene transfer in mouse neural precursors with a bicistronic retroviral vector.
Journal of neuroscience research - 1 Aug 2001
Franceschini I A, Feigenbaum-Lacombe V, Casanova P, Lopez-Lastra M, Darlix J L, Dalcq M D
Abstract excerpt
Gene transfer into neural precursors is a powerful approach to study the function of specific gene products during nervous system development. Here we describe a retrovirus-based methodology to transduce foreign genes into mouse neural precursors. We used a high-titer bicistronic retroviral vector that encodes a marker gene, placental alkaline phosphatase (plap), and a selection gene, neomycin phosphotransferase...
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