Article
Arabidopsis PTB1 and PTB2 proteins negatively regulate splicing of a mini-exon splicing reporter and affect alternative splicing of endogenous genes differentially.
The New phytologist - 1 Jul 2014
Simpson Craig G, Lewandowska Dominika, Liney Michele, Davidson Diane, Chapman Sean, Fuller John, McNicol Jim, Shaw Paul, Brown John W S
Abstract excerpt
This paper examines the function of Arabidopsis thaliana AtPTB1 and AtPTB2 as plant splicing factors. The effect on splicing of overexpression of AtPTB1 and AtPTB2 was analysed in an in vivo protoplast transient expression system with a novel mini-exon splicing reporter. A range of mutations in pyrimidine-rich sequences were compared with and without AtPTB and NpU2AF65 overexpression. Splicing analyses of...
Topics
- Alternative Splicing
- Arabidopsis
- Arabidopsis Proteins
- Carbohydrate Epimerases
- Exons
- Gene Expression Regulation, Plant
- Genes, Reporter
- Mutation
- Nuclear Proteins
- Plants, Genetically Modified
- RNA-Binding Proteins
- Ribonucleoproteins