Article
Probing the proton channel and the retinal binding site of Natronobacterium pharaonis sensory rhodopsin II.
Biophysical journal - 1 Apr 2002
Klare Johann P, Schmies Georg, Chizhov Igor, Shimono Kazumi, Kamo Naoki, Engelhard Martin
Abstract excerpt
The sensory rhodopsin II from Natronobacterium pharaonis (NpSRII) was mutated to try to create functional properties characteristic of bacteriorhodopsin (BR), the proton pump from Halobacterium salinarum. Key residues from the cytoplasmic and extracellular proton transfer channel of BR as well as from the retinal binding site were chosen. The single site mutants L40T, F86D, P183E, and T204A did not display...
Topics
- Alanine
- Archaeal Proteins
- Binding Sites
- Carotenoids
- Cell Membrane
- Cytoplasm
- DNA
- Escherichia coli
- Halorhodopsins
- Hydrogen-Ion Concentration
- Lasers
- Light
- Lipid Metabolism
- Models, Molecular
- Mutation