Article
New approaches for validation of lethal phenotypes and genetic reversion in Helicobacter pylori.
Helicobacter - 1 Mar 2001
McDaniel T K, Dewalt K C, Salama N R, Falkow S
Abstract excerpt
BACKGROUND: Because of limited genetic tools for use in Helicobacter pylori, tests routinely applied in other bacteria for demonstrating a gene's role in viability and other phenotypes have not been applied to this organism. In a mutational study of putative response regulator genes, we aimed to develop such tools for H. pylori. MATERIALS AND METHODS: We attempted to mutate five response regulator genes by...
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