Article
Evaluation of the homologous recombination in Helicobacter pylori.
Helicobacter - 1 Jun 2005
Pyndiah Slovénie, Ménard Armelle, Zerbib Frank, Mégraud Francis
Abstract excerpt
BACKGROUND: Most strategies for direct mutagenesis of Helicobacter pylori primarily involve genomic DNA cloning which is a time-consuming and expensive technique. METHODS: To make a gene replacement, we propose a strategy using polymerase chain reaction (PCR) amplicons to allow a double homologous recombination in the genome of H. pylori. Different strains were used to validate this strategy and we describe how...
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