HLA-B typing by reference strand mediated conformation analysis using a capillary-based semiautomated genetic analyzer.

doi:10.1016/s0198-8859(01)00213-0

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Article

HLA-B typing by reference strand mediated conformation analysis using a capillary-based semiautomated genetic analyzer.

Human immunology - 1 Apr 2001

Turner D, Akpe S, Brown J, Brown C, McWhinnie A, Madrigal A, Navarrete C

Abstract excerpt

The application of reference strand conformation analysis (RSCA) to HLA-A typing using the ABI PRISM 310 capillary based genetic analyzer has recently been described. This study outlines the development and validation of capillary RSCA for HLA-B typing. Mobility values for 93 HLA-B alleles were defined following electrophoresis of known controls through the system. Three fluorescently labelled references,...

Topics

Alleles
Automation
HLA-B Antigens
Histocompatibility Testing
Humans
Nucleic Acid Conformation
Polymerase Chain Reaction

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PMID: 11295475
DOI: 10.1016/s0198-8859(01)00213-0

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