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Article

Role of Lys-12 in catalysis by triosephosphate isomerase: a two-part substrate approach.

Biochemistry - 29 Jun 2010

Go Maybelle K, Koudelka Astrid, Amyes Tina L, Richard John P

Abstract excerpt

We report that the K12G mutation in triosephosphate isomerase (TIM) from Saccharomyces cerevisiae results in (1) a approximately 50-fold increase in K(m) for the substrate glyceraldehyde 3-phosphate (GAP) and a 60-fold increase in K(i) for competitive inhibition by the intermediate analogue 2-phosphoglycolate, resulting from the loss of stabilizing ground state interactions between the alkylammonium side chain of...

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Identifiers

PMID: 20481463
DOI: 10.1021/bi100538b
PMCID: PMC2890037

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Source receipt: pubmed, retrieved 7 Sept 2026.

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