Article
Detection of microsatellite instability by fluorescence multiplex polymerase chain reaction.
The Journal of molecular diagnostics : JMD - 1 Feb 2000
Berg K D, Glaser C L, Thompson R E, Hamilton S R, Griffin C A, Eshleman J R
Abstract excerpt
We have created a clinical molecular diagnostic assay to test for microsatellite instability (MSI) at multiple loci simultaneously in paraffin-embedded surgical pathology colon resection specimens. This fluorescent multiplex polymerase chain reaction (PCR) assay analyzes the five primary microsatellite loci recommended at the 1997 National Cancer Institute-sponsored conference on MSI for the identification of MSI...
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