Article
[Y97V substitution in the horse cytochrome c causes accumulation of the equilibrium intermediate].
Biofizika - 1 Jan 2000
Latypov R F, Dolgikh D A, Kirpichnikov M P, Ptitsyn O B, Roder H
Abstract excerpt
Equilibrium unfolding experiments on several mutant forms of horse heart cytochrome c were performed. By means of absorbance spectroscopy, the accumulation of an equilibrium intermediate was revealed upon unfolding of Y97V mutant protein, and its structural properties were characterized. The data obtained allow one to conclude that the equilibrium intermediate corresponds to the earliest kinetic intermediate Ic...
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