Article
[Development of a detection system (APC yeast color assay) of APC mutations by color change of yeast].
[Hokkaido igaku zasshi] The Hokkaido journal of medical science - 1 Nov 2000
Furuuchi K
Abstract excerpt
The author developed a sensitive yeast-based color assay which expresses APC-ADE2 (reporter) fusion protein in yeast and can screen almost the entire coding region of the APC gene. In this assay, the wild-type APC coding sequence of 8.5 kb is divided into 5 overlapping regions which are respectively ligated in-frame with an ADE2 open reading frame. The resulting five constructs containing a part of wild-type APC...
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