Article
Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: color-based selection of colonies using the met30 strain.
Journal of bioscience and bioengineering - 1 Dec 2012
Tarutina Marina G, Dutova Tatiana A, Yezhova Inna E, Nishiuchi Hiroaki, Sineoky Sergey P
Abstract excerpt
Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium w...
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