Article
Influence of preassay and sequence variations on viral load determination by a multiplex real-time reverse transcriptase-polymerase chain reaction for feline immunodeficiency virus.
Journal of acquired immune deficiency syndromes (1999) - 1 Jan 2001
Klein D, Leutenegger C M, Bahula C, Gold P, Hofmann-Lehmann R, Salmons B, Lutz H, Gunzburg W H
Abstract excerpt
Determination of retroviral load is an important tool in the investigation of the success of therapeutic or vaccination trials in patients infected with lentiviruses such as HIV, or with their simian (SIV) or feline (FIV) counterparts. We have developed an one-tube quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay based on the ABI Prism 7700 Sequence Detection System (TaqMan) to quantify...
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