Article
High-resolution melting analysis for simultaneous detection and discrimination between wild-type and vaccine strains of feline calicivirus.
The veterinary quarterly - 1 Dec 2023
Phongroop Kannika, Rattanasrisomporn Jatuporn, Tangtrongsup Sahatchai, Rungsipipat Anudep, Piewbang Chutchai, Techangamsuwan Somporn
Abstract excerpt
High-resolution melting (HRM) analysis, a post-polymerase chain reaction (PCR) application in a single closed tube, is the straightforward method for simultaneous detection, genotyping, and mutation scanning, enabling more significant dynamic detection and sequencing-free turnaround time. This study aimed to establish a combined reverse-transcription quantitative PCR and HRM (RT-qPCR-HRM) assay for diagnosing and...
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