Article
Mouse cloned from embryonic stem (ES) cells synchronized in metaphase with nocodazole.
The Journal of experimental zoology - 1 Feb 2001
Amano T, Tani T, Kato Y, Tsunoda Y
Abstract excerpt
Full-term development occurred when nuclei from mouse embryonic stem (ES) cells, synchronized in metaphase with nocodazole, were fused with enucleated oocytes or nuclei of reconstituted eggs and again fused with the enucleated blastomeres of fertilized two-cell embryos using inactivated Sendai virus. Two surviving male mice were derived from undifferentiated ES cell nuclei, one from single nuclear transfer and...
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