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Mouse cloning with nucleus donor cells of different age and type

1 Jan 2001

Abstract excerpt

We have tested different cell types as sources for nucleus donors to determine differences in cloning efficiency. When donor nuclei were isolated from cumulus cells and injected into recipient oocytes from adult hybrid mice (B6D2F1 and B6C3F1), the success rate of cloning was 1.5-1.9%. When cumulus cell donor nuclei were isolated from adult inbred mice (C57BL/6, C3H/He, DBA/2, 129/SvJ, and 129/SvEvTac),...

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PMID: 11241773
DOI: 10.1002/1098-2795(20010401)58:4<376::aid-mrd4>3.0.co;2-l

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