Article
Induction of antigen specific CD4+ T cell responses by invariant chain based DNA vaccines.
Vaccine - 8 Jan 2001
van Tienhoven E A, ten Brink C T, van Bergen J, Koning F, van Eden W, Broeren C P
Abstract excerpt
In this report, the use of DNA vaccination to induce class II restricted antigen specific proliferative responses was studied. To this end, a construct encoding the invariant chain (Ii) was engineered in which the Class II associated invariant chain peptide (CLIP) sequence was replaced by an immunogenic epitope derived form Heat Shock Protein 60, HSP60 178-186. Transfection studies in vitro showed that this...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Differentiation, B-Lymphocyte
- Base Sequence
- CD4-Positive T-Lymphocytes
- COS Cells
- Chaperonin 60
- DNA, Recombinant
- Histocompatibility Antigens Class II
- In Vitro Techniques
- Lymphocyte Activation
- Male
- Molecular Sequence Data
- Mutation
- Rats
- Rats, Inbred Lew
- Transfection
- Vaccines, DNA