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Reverse Genetics System for Uukuniemi Virus ( <i>Bunyaviridae</i> ): RNA Polymerase I-Catalyzed Expression of Chimeric Viral RNAs

15 Feb 2001

Abstract excerpt

We describe here the development of a reverse genetics system for the phlebovirus Uukuniemi virus, a member of the Bunyaviridae family, by using RNA polymerase I (pol I)-mediated transcription. Complementary DNAs containing the coding sequence for either chloramphenicol acetyltransferase (CAT) or green fluorescent protein (GFP) (both in antisense orientation) were flanked by the 5'- and 3'-terminal untranslated...

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Identifiers

PMID: 11160662
DOI: 10.1128/jvi.75.4.1643-1655.2001

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