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Article

Differential fluorescence induction reveals <i>Streptococcus pneumoniae</i> loci regulated by competence stimulatory peptide

1 Jan 2001

Abstract excerpt

Differential fluorescence induction (DFI) in Streptococcus pneumoniae was used as a method for the discovery of genes activated in specific growth environments. Competence stimulatory peptide (CSP) was used as the model inducing system to identify differentially expressed genes. To identify CSP-induced promoters, a plasmid library was constructed by inserting random pieces of S. pneumoniae chromosomal DNA...

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PMID: 11123694
DOI: 10.1046/j.1365-2958.2001.02218.x

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