Article
Genetic and biochemical characterization of a protein phosphatase with dual substrate specificity in Streptomyces coelicolor A3(2).
Gene - 27 Nov 2000
Umeyama T, Naruoka A, Horinouchi S
Abstract excerpt
A gene encoding a protein phosphatase (SppA) with a phosphoesterase motif, which was predicted by the genome project of the Gram-positive bacterium Streptomyces coelicolor A3(2), was cloned by PCR in pET32a(+) and expressed in Escherichia coli. SppA fused to thioredoxin (TRX-SppA) showed distinct heat-stable phosphatase activity toward p-nitrophenyl phosphate with optimal pH 8.0 and optimal temperature 55 degrees...
Topics
- Amino Acid Sequence
- Cell Division
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Enzyme Inhibitors
- Enzyme Stability
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Hydrogen-Ion Concentration
- Metals
- Microscopy, Electron, Scanning
- Molecular Sequence Data