Article
Expression of the Streptomyces coelicolor A3(2) ptpA gene encoding a phosphotyrosine protein phosphatase leads to overproduction of secondary metabolites in S. lividans.
FEMS microbiology letters - 1 Nov 1996
Umeyama T, Tanabe Y, Aigle B D, Horinouchi S
Abstract excerpt
A DNA fragment that caused pigment production in Streptomyces lividans was isolated from a gene library of PstI-digested chromosomal fragments of S. coelicolor A3(2). Subcloning and nucleotide sequencing proved the identity of the cloned gene to ptpA encoding a low-molecular-mass phosphotyrosine...
Topics
- 4-Butyrolactone
- Anthraquinones
- Bacterial Proteins
- Cloning, Molecular
- DNA, Bacterial
- Enzyme Induction
- Gene Expression Regulation, Bacterial
- Gene Library
- Gene Targeting
- Genes, Bacterial
- Phenotype