Article
Differential processing of UV mimetic and interstrand crosslink damage by XPF cell extracts.
Nucleic acids research - 1 Dec 2000
Zhang N, Zhang X, Peterson C, Li L, Legerski R
Abstract excerpt
We have recently developed a mammalian cell free assay in which interstrand crosslinks induce DNA synthesis in both damaged and undamaged plasmids co-incubated in the same extract. We have also shown using hamster mutants that both ERCC1 and XPF are required for the observed incorporation. Here, we show that extracts from an XPF patient cell line differentially process UV mimetic damage and interstrand crosslinks...
Topics
- Animals
- CHO Cells
- Cell Line
- Cell Line, Transformed
- Cell Survival
- Cell-Free System
- Cricetinae
- Cross-Linking Reagents
- DNA
- DNA Damage
- DNA Repair
- DNA-Binding Proteins
- Dose-Response Relationship, Drug
- Endonucleases
- Humans
- Mitomycin
- Mutation
- Proteins