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Development of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains.

Journal of clinical microbiology - 1 Sept 2009

Ooka Tadasuke, Terajima Jun, Kusumoto Masahiro, Iguchi Atsushi, Kurokawa Ken, Ogura Yoshitoshi, Asadulghani Md, Nakayama Keisuke, Murase Kazunori, Ohnishi Makoto, Iyoda Sunao, Watanabe Haruo, Hayashi Tetsuya

Abstract excerpt

Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods is crucial for the rapid detection and surveillance of EHEC O157 outbreaks. In the present study, we developed a multiplex PCR-based strain-typing method for EHEC O157, which is based on the variability in genomic location of IS629...

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Identifiers

PMID: 19641072
DOI: 10.1128/JCM.00792-09
PMCID: PMC2738077

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