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Article

Rational design and purification of human Bruton's tyrosine kinase SH3-SH2 protein for structure-function studies.

Protein expression and purification - 1 Dec 2000

Nera K P, Brockmann E, Vihinen M, Smith C I, Mattsson P T

Abstract excerpt

Bruton's tyrosine kinase (Btk) is a cytoplasmic protein tyrosine kinase consisting of N-terminal pleckstrin homology (PH) domain followed by Tec homology (TH) domain, Src homology 3 and 2 (SH3 and SH2) domains, and a C-terminal kinase domain. Mutations in the human BTK gene cause the severe immunodeficiency disease X-linked agammaglobulinemia (XLA). The structural and functional basis of several XLA-causing...

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Source

Identifiers

PMID: 11087675
DOI: 10.1006/prep.2000.1316

Genes

BTK

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Rational design and purification of human Bruton's tyrosine kinase SH3-SH2 protein for structure-function studies.PMID 11087675Read ↗

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Bruton tyrosine kinase (BTK) in X-linked agammaglobulinemia (XLA).Semantic proximity Read ↗

Btk SH2-kinase interface is critical for allosteric kinase activation and its targeting inhibits B-cell neoplasmsSemantic proximity Read ↗

Identification of Bruton's tyrosine kinase (Btk) gene mutations and characterization of the derived proteins in 35 X-linked agammaglobulinemia families: a nationwide study of Btk deficiency in Japan.Semantic proximity Read ↗

A regulatory SH2 domain-targeting protein binder effectively inhibits the activity of Bruton's tyrosine kinase and its drug-resistant variants.Semantic proximity Read ↗

Structure of the PH domain and Btk motif from Bruton's tyrosine kinase: molecular explanations for X-linked agammaglobulinaemia.Semantic proximity Read ↗

Molecular and structural characterization of five novel mutations in the Bruton's tyrosine kinase gene from patients with X-linked agammaglobulinemia.Semantic proximity Read ↗

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ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library.Semantic proximity Read ↗

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Engineered antibody CH2 domains binding to nucleolin: Isolation, characterization and improvement of aggregation.Semantic proximity Read ↗

Rescue of the aggregation prone Itk Pleckstrin Homology domain by two mutations derived from the related kinases, Btk and Tec.Semantic proximity Read ↗

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