Article
Detection and Quantification of Plasmodium falciparum in Blood Samples Using Quantitative Nucleic Acid Sequence-Based Amplification
1 Nov 2000
Abstract excerpt
A quantitative nucleic acid sequence-based amplification (QT-NASBA) assay for the detection of Plasmodium parasites has been developed. Primers and probes were selected on the basis of the sequence of the small-subunit rRNA gene. Quantification was achieved by coamplification of the RNA in the sample with one modified in vitro RNA as a competitor in a single-tube NASBA reaction. Parasite densities ranging from 10...
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