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Article

Phage phi 29 DNA polymerase residues involved in the proper stabilisation of the primer-terminus at the 3'-5' exonuclease active site.

Journal of molecular biology - 17 Nov 2000

de Vega M, Lázaro J M, Salas M

Abstract excerpt

Three highly conserved amino acid residues have been characterised here as ssDNA ligands at the 3'-5' exonuclease active site of o29 DNA polymerase. The functional role of Tyr59, His61 and Phe69 residues of o29 DNA polymerase (belonging to Exo II motif, previously described as containing an invariant catalytic aspartate residue and two highly conserved ssDNA ligands) was assayed by biochemical analysis of six...

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Identifiers

PMID: 11071805
DOI: 10.1006/jmbi.2000.4178

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